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Adenine

CAS Number73-24-5
Molecular FormulaC5H5N5
Molecular Weight135.131 g/mol
InChI KeyGFFGJBXGBJISGV-UHFFFAOYSA-N
LogP-0.0900
Synonyms
  • Adenine
  • 9H-Purin-6-amine
  • 73-24-5
  • 1H-Purin-6-amine
  • 1,6-Dihydro-6-iminopurine
  • 3,6-Dihydro-6-iminopurine
  • 3H-Purin-6-amine
  • 6-Amino-1H-purine
  • 6-Amino-3H-purine
  • 6-Amino-9H-purine
  • 6-Aminopurine
  • 7H-Purin-6-amine
  • 9H-Purin-6-amine
  • adenina
  • Adeninimine
  • NSC 14666
  • Pedatisectine B
  • EINECS 200-796-1
  • 1H-Purine, 6-amino
  • Purine, 6-amino-
  • 9H-Purine, 1,6-dihydro-6-imino-
  • UNII-JAC85A2161
  • 1H-Purine-6-amine
  • 6-Amino-7H-purine
  • 6-Amino-Purine
  • 9H-Purin-6-yl-amin
  • 9H-Purin-6-ylamine
  • 9H-Purine-6-amine
  • Ade
  • Adenin
  • Vitamin B4
  • 1000264-10-7
  • 22051-90-7
  • 42911-33-1
  • 42911-34-2
  • 520-75-2
  • 66224-65-5
  • 71660-29-2
  • 71660-30-5

Applications:

HPLC Application for Separation of Nucleotide Bases

Nucleotide bases are parts of DNA and RNA. Adenine and guanine are purine-bases; uracil, thymine and cytosine are pyrimidine-bases. In the view of chromatography these compounds are very polar and similar in properties. It is hard to obtain base line HPLC separation on traditional C18 as peaks of nucleotide bases co-elute even at low organic concentration. In this application nucleobases are well retained and separated on Primesep 200 mixed-mode column. Compounds are retained by weak reverse phase and weak ion-exchange mechanisms. This HPLC method can utilize UV, ELSD, and LC/MS detection.
 

Condition

ColumnPrimesep 200, 4.6*250 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 10/90%
BufferTFA – 0.2%
Flow Rate0.5 ml/min, 1.0 ml/min
DetectionUV, 270 nm

Description

Class of CompoundsDrug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing CompoundsUracil, Thymine, Cytosine, Guanine, Adenine