Adenine

CAS Number	73-24-5
Molecular Formula	C5H5N5
Molecular Weight	135.131 g/mol
InChI Key	GFFGJBXGBJISGV-UHFFFAOYSA-N
LogP	-0.0900
Synonyms	Adenine 9H-Purin-6-amine 73-24-5 1H-Purin-6-amine 1,6-Dihydro-6-iminopurine 3,6-Dihydro-6-iminopurine 3H-Purin-6-amine 6-Amino-1H-purine 6-Amino-3H-purine 6-Amino-9H-purine 6-Aminopurine 7H-Purin-6-amine 9H-Purin-6-amine adenina Adeninimine NSC 14666 Pedatisectine B EINECS 200-796-1 1H-Purine, 6-amino Purine, 6-amino- 9H-Purine, 1,6-dihydro-6-imino- UNII-JAC85A2161 1H-Purine-6-amine 6-Amino-7H-purine 6-Amino-Purine 9H-Purin-6-yl-amin 9H-Purin-6-ylamine 9H-Purine-6-amine Ade Adenin Vitamin B4 1000264-10-7 22051-90-7 42911-33-1 42911-34-2 520-75-2 66224-65-5 71660-29-2 71660-30-5

Applications:

HPLC Application for Separation of Nucleotide Bases

Nucleotide bases are parts of DNA and RNA. Adenine and guanine are purine-bases; uracil, thymine and cytosine are pyrimidine-bases. In the view of chromatography these compounds are very polar and similar in properties. It is hard to obtain base line HPLC separation on traditional C18 as peaks of nucleotide bases co-elute even at low organic concentration. In this application nucleobases are well retained and separated on Primesep 200 mixed-mode column. Compounds are retained by weak reverse phase and weak ion-exchange mechanisms. This HPLC method can utilize UV, ELSD, and LC/MS detection.

Condition

Column	Primesep 200, 4.6*250 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O – 10/90%
Buffer	TFA – 0.2%
Flow Rate	0.5 ml/min, 1.0 ml/min
Detection	UV, 270 nm

Description

Class of Compounds	Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds	Uracil, Thymine, Cytosine, Guanine, Adenine